By Stephen E. Harding, Michael P. Tombs, Gary G. Adams, Berit Smestad Paulsen, Kari Tvete Inngjerdingen, Hilde Barsett
Polysaccharides and comparable excessive molecular weight glycans are highly various with huge software in Biotechnology and nice possibilities for additional exploitation. An creation to Polysaccharide Biotechnology – a moment version of the preferred unique textual content through Tombs and Harding – introduces scholars, researchers, clinicians and industrialists to the houses of a few of the major fabrics concerned, how those are utilized, many of the fiscal components relating their construction and the way they're characterised for regulatory purposes.
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Additional resources for An Introduction to Polysaccharide Biotechnology
3 Mass per Unit Lengths, ML, and Persistence Lengths, Lp, of Some Glycopolymers. 4 Conformation Zones of Polysaccharides Conformation Zone Nature Examples Schizophyllan, scleroglucan Xanthan D Rigid rod Rigid rod with limited flexibility Semi-flexible coil or asymmetric coil Random coil E Globular or highly branched A B C Alginate, pectin, chitosan, xylans, cellulose nitrate, methylcellulose Pullulan, dextran, guar, locust-bean gum, konjac mannan Amylopectin Chapter 1 – Polysaccharides and Their Potential for Biotechnology 21 Formulae exist relating Rg and other hydrodynamic data to the axial ratio of rods and other rigid-structure models for polysaccharide conformation such as ellipsoids and bead models, but this is beyond the scope of this book.
Conversely, in other parts of the world advances in agricultural practice may lead to the appearance of novel raw materials, which must be evaluated. Another source of new raw materials is the discovery in some remote part of the world (that is to say remote from the manufacturer) of a material which, while well known to the locals, has obvious potential in some other context. There are many examples in the polysaccharide field. The Japanese or the Welsh, who were using seaweed, alginates and their derivative products centuries ago, did not imagine that they would be useful in making ice cream.
If a DNA sequence encoding the enzyme is available from a suitable search of the public nucleotide databases, the entire amino acid sequence of the protein can be readily obtained. This of course depends on there being DNA sequence information available on your organism of interest. This is why biochemical and molecular studies are usually best undertaken on an organism where the genome sequence is readily available. In practice, the purified enzyme is needed for a few other purposes as well as sequencing.
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